Signalling Functions of TAMPs at Epithelial Tight Junctions

I completed my project on the signalling functions of tight junction proteins at epithelial tight junctions under the supervision of Prof. Karl Matter, at UCL's Institute of Ophthalmology.

Tight junctions are important protein complexes found between epithelial cells. They both maintain cellular barriers and control cell differentiation, proliferation, and migration through various signalling pathways. These pathways are important in cellular differentiation, and can be deregulated in epithelial cancers and other diseases. 

I investigated how structural domains of a tight junction-associated MARVEL protein (TAMP) regulates cell morphology and cytoskeletal organisation, through biochemical techniques and immunofluorescence microscopy. This project involved training in recombinant protein expression, mammalian cell culture, pulldown assays and immunoblotting, and immunofluorescence microscopy.

This was part of an ongoing project in the lab to further characterise the the role of cell-cell adhesion proteins in regulation of epithelial cell behaviour and differentiation.